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KMID : 0613820090190030366
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Journal of Life Science
2009 Volume.19 No. 3 p.366 ~ p.372
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Amylase Activity and Characterization of Microorganism Isolated from in Aquacultural Effluents Sediment Layer
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Kim Man-Chul
Moon Yonng-Gun
Heo Moon-Soo
Song Chang-Young
Kim Gi-Young
Jang Tae-Won
Harikrishnan Ramasamy
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Abstract
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In the course of screening of useful enzyme-producing microorganisms from marine sedimentary layers, we isolated 2 amylase producing strains and tested their amylase producing activities. Analyses of 16S rDNA sequences and biochemical methods (BIOLOG) of two isolates showed that they were confirmed to be a gram positive Bacillus sp. and gram negative Pseudoalteromonas sp., respectively. Excellent amylase producing strains were termed Bacillus sp. ST-63 and Pseudoalteromonas sp. ST-140, and further studies were conducted on their amylase producing characteristics. Optimum conditions for cell growth in amylase activity were obtained when the isolate (Bacillus sp. ST-63 and Pseudoalteromonas sp. ST-140) was cultured at 30℃ and pH 7～8.
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KEYWORD
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Amlyase, 16S rDNA, BIOLOG, Bacillus sp., Pseudoalteromonas sp
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